GÉTICA 2021

47 [ F I T C á n c e r - 7 ] dified variable and constant region sequen- ces. Though this conference we demonstrate how stiTChR can process a million TCRs in under 15 minutes on a desktop computer. Additionally, by synthesizing sequences pro- duced by stiTChR and transducing them into Jurkat cells we show that these sequences recapitulate the reported antigen specifici- ty of the parent receptor. By systemizing the production and modification of TCR sequen- ces, we propose that stiTChR will increase the speed, repeatability, and reproducibility of TCR research (Figs. 1-3). In literature and in data receptors TCRs, pro- duced by their VDJ gene recombination, are referenced by their combination of their V and J genes names plus the hypervariable aCDR3 amino acid sequence. To reproduce the exact TCR sequence, a complete coding nucleotide sequence is required. Here we present stiTChR software that will fill this fun- damental problemby reproducing TCRs. This can produce complete and valid sequences representative of a fully spliced TCR cDNA gi- ven minimal V/D/CDR3 information. It can be used for TCR engineering, swapping in mo- CC-10. STITCHR: STITCHING CODING TCR NUCLEOTIDE SEQUENCES FROM V/J/CDR3 INFORMATION Heather, Jamie M.; Spindler, Matthew; Herrero Alonso, Marta; Shui, Yifang; Millar, David G.; Johnson, David S.; Cobbold, Mark; Hata, Aaron Massachusetts General Hospital Cancer Center. Massachusetts, United States Figure 1.