GÉTICA 2021

56 VII FORO DE I nmunología Traslacional e INMUNOTERAPIA DEL CÁNCER Figure 3. CAR-T19 cells induced CD19 down-modulation and degradation. NALM6 cells were co-cultured for 2 hours at a 1:1 E:T ratio with non-transduced Jurkat cells (J-NT), J-CAR-T19 or J-STAb-T19 cells. (a) Representative dot plots showing CD2, CD19 and CD10 expression. (b) Analysis of CAR down-modulation in J-CAR-T19 cells in co-cultures with NALM6 cells. (c) Representa- tive images of CD19 and LAMP1 cellular localization in NALM6 cells co-cultured with J-NT-T, J-CAR-T19 or J-STAb-T19 cells. (d) Pearson’s coefficients’ for CD19 and LAMP1 colocalization assessment of NALM6 entire cells in the indicated co-cultures. Dots represent the analyzed cells in one experiment representative of two done. Mean ± SD values are shown. The p values of the one-way ANOVA with Tukey’s multiple comparison tests are shown.